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Merck

SAB4200689

Sigma-Aldrich

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal

clone 5C5, hybridoma cell culture supernatant

Synonym(e):

Actin alpha sarcomeric/skeletal, a-SCA, alpha-SCA, alpha-sarcomeric actin

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

5C5, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~42 kDa

Speziesreaktivität

rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus

Verpackung

antibody small pack of 25 μL

Methode(n)

ELISA: suitable
immunoblotting: 1:500-1:1000 using rat heart tissue extracts
immunofluorescence: 1:500-1000 using human HeLa cells.
immunohistochemistry: 1:500 using formalin-fixed, paraffin-embedded human tongue sections and Biotin/ExtrAvidin®-Peroxidase staining system.

Isotyp

IgM

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... ACTA2(59)

Allgemeine Beschreibung

Monoclonal Anti-Actin (-Sarcomeric) (mouse IgM isotype) is derived from the hybridoma 5C5 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified rabbit striated muscle. Actin is the major cytoskeletal protein in eukaryotic cells.

Immunogen

purified rabbit striated muscle

Anwendung

Anti-Actin (α-Sarcomeric) antibody, Mouse monoclonal has been used in:
  • indirect immunofluorescence staining
  • immunohistochemistry
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunofluorescence

Biochem./physiol. Wirkung

Actin plays essential roles in a number of cellular processes including cell migration, cytokinesis, vesicle transport and contractile force generation.

Physikalische Form

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.

Rechtliche Hinweise

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles
Miklavc P, et al.
Journal of Cell Science, 128(6), 1193-1203 (2015)
Clarkson, E., et al.,
The Journal of Pathology, 204, 407-417 (2004)
Babai, F., et al.,
Virchows Archiv. B, Cell Pathology Including Molecular Pathology, 55, 263-277 (1988)
PEDF decreases cardiomyocyte edema during oxygen-glucose deprivation and recovery via inhibiting lactate accumulation and expression of AQP1
Huang B, et al.
International Journal of Molecular Medicine, 43(5), 1979-1990 (2019)
The actin cytoskeleton
Molecular and Cellular Biology, 1979-1990 (2000)

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