SAB4200646

Monoclonal Anti-ALIX/PDCD6IP antibody produced in mouse

clone ALIX-1, purified from hybridoma cell culture

• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: ALIX-1, monoclonal
• Form: buffered aqueous solution
• Speziesreaktivität: human
• Konzentration: ~1 mg/mL
• Methode(n): flow cytometry: 5-10 μg/test using using human MOLT4 cells; immunoblotting: 2-5 μg/mL using using whole extracts of human MOLT4 cells.; immunofluorescence: 10-20 μg/mL using using human HeLa cells; immunoprecipitation (IP): 10-20 μg/mL using using lysates of human MOLT4 cells.
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: Q8WUM4
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PDCD6IP(10015)

Beschreibung

Allgemeine Beschreibung

Anti-ALIX/PDCD6IP antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ALIX-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X), also named Programmed Cell Death 6 interacting protein (PDCD6IP).

Immunogen

peptide corresponding to an internal region of human ALIX/ PDCD6IP

Anwendung

Monoclonal Anti-ALIX/PDCD6IP antibody has been used in

• immunoblotting
• immunoprecipitation
• immunofluorescence
• flow cytometry

Biochem./physiol. Wirkung

ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X) plays a role in ESCRT (endosomal sorting complexes required for transport) pathway. It is involved in the abscission stage of cytokinesis, in intralumenal endosomal localization, and in enveloped virus budding. Overexpression of this protein can block apoptosis. In addition, ALIX binds to PDCD6, a protein required for apoptosis, in a calcium-dependent manner. ALIX also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable