F1180

Anti-Flotillin 1 in Kaninchen hergestellte Antikörper

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-FLOT1, Anti-Reggie-2
• MDL-Nummer: MFCD01633353
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: ~48 kDa
• Speziesreaktivität: rat, mouse, human
• Konzentration: ~1 mg/mL
• Methode(n): indirect immunofluorescence: 5-10 μg/mL using human HEK-293K cells; western blot: 0.5-1 μg/mL using whole extract of mouse lung; western blot: 1-2 μg/mL using whole extract of rat lung
• UniProt-Hinterlegungsnummer: O75955
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FLOT1(10211) ; mouse ... Flot1(14251) ; rat ... Flot1(64665)

Beschreibung

Allgemeine Beschreibung

Flotillin 1(FLOT1), also known as reggie-2, is an estrogen responsive gene mapped to human chromosome 6p21.33. This gene codes for an integral membrane protein and a component of lipid rafts.

Flotillins are lipid raft associated proteins expressed mainly in adipose tissue, striated muscle tissues, and lung. It has a crucial role in several cellular processes like phagocytosis, endocytosis, insulin signalling and neuronal regeneration. Anti-flotillin 1 antibody can be used in immunobloting and immunolocalization. Rabbit anti- flotillin 1 antibody reacts specifically with flotillin 1 of rat, mouse and human.

Immunogen

synthetic peptide corresponding to amino acid resides 413-428 of mouse flotillin 1 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and differs by one amino acid in human.

Anwendung

Anti-flotillin 1 antibody can be used in immunofluorescence and western blotting.

Anti-flotillin 1 antibody produced in rabbit has been used in immunoblot analysis.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Biochem./physiol. Wirkung

Flotillin 1 (FLOT1) has a role in endocytosis. It also functions in growth factor signaling and activates mitogen-activated protein kinase signaling. In addition, it is also involved in phagocytosis, regulation of actin cytoskeleton and membrane trafficking. The protein has been associated with tumor progression and is expressed in many cancers, including breast cancer.

Zielbeschreibung

Flotillin 1 encodes a caveolae-associated, integral membrane protein. Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)