Direkt zum Inhalt
Merck

SAB4200543

Sigma-Aldrich

Anti-Calbindin-D-28K antibody, Mouse monoclonal

enhanced validation

clone CB-955, purified from hybridoma cell culture

Synonym(e):

Monoclonal Anti-(28kD), Monoclonal Anti-D-28K, Monoclonal Anti-D-dependent calcium-binding protein, Monoclonal Anti-RTVL-H protein, Monoclonal Anti-avian-type, Monoclonal Anti-calbindin 1, Monoclonal Anti-vitamin

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203
NACRES:
NA.44

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

CB-955, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~28 kDa

Speziesreaktivität

mouse, human, rat

Erweiterte Validierung

independent
Learn more about Antibody Enhanced Validation

Konzentration

~1.0 mg/mL

Methode(n)

immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin embedded rat cerebellum.
western blot: 2-4 μg/mL using MDBK extract.

Isotyp

IgG1

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CALB1(793)

Allgemeine Beschreibung

Anti-Calbindin-D-28K antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CB-955 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified bovine kidney calbindin-D-28K.
Calbindin-D-28K (CALB1) is encoded by the gene mapped to human chromosome 8q21.3-q22.1. CALB1 belongs to the EF hand superfamily of Ca2+ binding proteins (CBPs). The encoded protein contains EF hand motif involved in the specific and reversible binding of Ca2+. It is highly expressed in specific regions of central nervous system such as cerebellar Purkinje cells and hippocampal granule cells.

Immunogen

purified bovine kidney calbindin-D-28K. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.

Anwendung

  • Anti-Calbindin-D-28K antibody, Mouse monoclonal has been used in:
  • western blotting
  • enzyme-linked immunosorbent assay (ELISA){31
  • immunohistochemistry
  • immunohistochemical procedure
  • indirect immunofluorescence

Biochem./physiol. Wirkung

Calbindin D-28k might play a vital role as an intracellular Ca2+ buffer. It has an ability to protect nerve cells from calcium-mediated neurotoxicity. The encoded protein functions as a potent neuroanatomical marker which can be used to selectively visualize certain neurons and pathways in the central nervous system and peripheral nervous system. Mutations in the gene has been observed in patients with Parkinson′s disease (PD).
Calbindin-D (28K) functions as a mobile or partly immobilized Ca2+ -buffer with medium kinetics and affinity buffer function. Second, it functions in buffered Ca2+- diffusion-transport function. Finally, it interacts with target proteins in its apo-and Ca2+-loaded form-sensor- like function. Over-expression of this protein stimulates neurite outgrowth in dopaminergic neuronal cells.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sie haben nicht das passende Produkt gefunden?  

Probieren Sie unser Produkt-Auswahlhilfe. aus.

Empfehlung

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

A comparative study of three interneuron types in the rat spinal cord
Chen S, et al.
PLoS ONE, 11(9), e0162969-e0162969 (2016)
The human calbindin D28k (CALB1) and calretinin (CALB2) genes are located at 8q21.3----q22.1 and 16q22----q23, respectively, suggesting a common duplication with the carbonic anhydrase isozyme loci.
Parmentier M
Cytogenetics and Cell Genetics, 57, 41-43 (1991)
Binding Kinetics of Calbindin-D28k Determined by Flash Photolysis of Caged Ca2+
Nagerl UV
Biophysical Journal, 79, 3009-3018 (2000)
A splice site variant in INPP5E causes diffuse cystic renal dysplasia and hepatic fibrosis in dogs
Dillard KJ, et al.
PLoS ONE, 13(9) (2018)
Calbindin 1, fibroblast growth factor 20, and ?-synuclein in sporadic Parkinson?s disease
Mizuta I
Human Genetics, 124, 89-94 (2008)

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.