S6147
Sephadex® G-100
DNA grade, BioReagent, for molecular biology, medium
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About This Item
Empfohlene Produkte
Qualität
for molecular biology
Produktlinie
BioReagent
Form
beads
Methode(n)
buffer exchange: suitable
Aktive Matrixgruppe
phase
Quellen
1 g swells to 9-11 mL
Perlendurchmesser
40-120 μm
Porengröße
exclusion limit (25 bp dsDNA; (rA)27)
pH-Wert
2-10
Anwendung(en)
life science and biopharma
Kompatibilität
Cytiva
Fremdaktivität
DNase, RNase, none detected
Lagertemp.
room temp
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Allgemeine Beschreibung
Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.
Anwendung
Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.
Leistungsmerkmale und Vorteile
- Excellent recovery
- Minimal sample dilution
- Quick and easy to use
Rechtliche Hinweise
Sephadex is a registered trademark of Cytiva
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Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
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