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S5772

Sigma-Aldrich

Sephadex® G-25

BioReagent, for molecular biology, DNA grade, Superfine

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About This Item

CAS-Nummer:
9041-35-4
MDL-Nummer:
MFCD00132770
UNSPSC-Code:
23151817
NACRES:
NA.52

Qualität

DNA grade
for molecular biology

Qualitätsniveau

200

Produktlinie

BioReagent

Form

beads

Methode(n)

buffer exchange: suitable

Aktive Matrixgruppe

phase

Perlendurchmesser

20-50 μm

Porengröße

exclusion limit (10 bp dsDNA; (rA)10)

pH-Bereich

2-13

Anwendung(en)

life science and biopharma

Kompatibilität

Cytiva

Fremdaktivität

DNase, RNase, none detected

Lagertemp.

room temp

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1

InChIKey

BCPVZFFJGCURNR-OCOFDJSDSA-N

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Allgemeine Beschreibung

Sephadex® G-25, Superfine is an often-cited gel filtration medium for desalting and buffer exchange. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Superfine” indicates the smallest particle size available.

Anwendung

Sephadex® G-25 is suitable for buffer exchange and desalting oligonucleotides for use in sequencing, PCR, and labeling applications. It was used to isolate two different peptides of Pronase E extracted from Alaska Pollack skin.

Leistungsmerkmale und Vorteile

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Rechtliche Hinweise

Sephadex is a registered trademark of Cytiva

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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GE17-0033-01

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GE17-0034-01

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Sephadex® G-15 Medium

Sigma-Aldrich

G15120

Sephadex® G-15

Sephadex® G-50 Superfine

Sigma-Aldrich

G5050

Sephadex® G-50

Microspin™ G-25-Säulen Cytiva 27-5325-01

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Microspin™ G-25-Säulen

S K Kim et al.
Journal of agricultural and food chemistry, 49(4), 1984-1989 (2001-04-20)
Gelatin extracted from Alaska pollack skin was hydrolyzed with serial digestions in the order of Alcalase, Pronase E, and collagenase using a three-step recycling membrane reactor. The fraction from the second step, which was hydrolyzed with Pronase E, was composed
Veronica Murta et al.
Journal of neurochemistry, 144(6), 748-760 (2017-12-28)
Secondary neuronal death is a serious stroke complication. This process is facilitated by the conversion of glial cells to the reactive pro-inflammatory phenotype that induces neurodegeneration. Therefore, regulation of glial activation is a compelling strategy to reduce brain damage after
Veronika Horkova et al.
Cell reports, 30(5), 1504-1514 (2020-02-06)
Overtly self-reactive T cells are removed during thymic selection. However, it has been recently established that T cell self-reactivity promotes protective immune responses. Apparently, the level of self-reactivity of mature T cells must be tightly balanced. Our mathematical model and experimental data
Nerine T Joseph et al.
The Journal of endocrinology, 202(1), 179-190 (2009-04-22)
Two GnRH isoforms (cGnRH-I and GnRH-II) and two GnRH receptor subtypes (cGnRH-R-I and cGnRH-R-III) occur in chickens. Differential roles for these molecules in regulating gonadotrophin secretion or other functions are unclear. To investigate this we cloned cGnRH-R-III from a broiler
Fernanda O Lemos et al.
European journal of dermatology : EJD, 21(5), 722-730 (2011-07-09)
Previous studies demonstrated that proteinases from latex of C. candamarcensis act as mitogens on fibroblast and epithelial cells and a subsequent report showed their protective, angiogenic and wound healing effects on gastric ulcers. In this study, we present evidence of
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