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Merck

S3396

Sigma-Aldrich

Monoclonal Anti-Spectrin (α and β) antibody produced in mouse

clone SB-SP1, ascites fluid

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Konjugat

unconjugated

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

SB-SP1, monoclonal

Enthält

15 mM sodium azide

Speziesreaktivität

human

Methode(n)

indirect immunofluorescence: suitable using erythrocyte smears
microarray: suitable
western blot: 1:500 using extract of human erythrocyte ghosts or purified human erythrocyte spectrin

Isotyp

IgG1

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

Allgemeine Beschreibung

Monoclonal anti-Spectrin (α and β) (mouse IgG1 isotype) is produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass.
Spectrins are heterotetramers, that are composed of several αand β subunit isoforms. They are the constituents of G-protein-coupled receptor (GPCR) and the synaptic multiprotein complex. α-Spectrin has 20 spectrin repeats (α1-α20) and β-spectrin has 17 spectrin repeats (β1-β17). SPTA1 (spectrin α 1) is located on chromosome 1q21–q23 and SPTB (spectrin β) is located on chromosome 14q22–q23.2.

Spezifität

This antibody reacts specifically with the α and β chains of human erythrocyte spectrin.

Immunogen

human erythrocyte spectrin.

Anwendung

Anti-Spectrin (α and β) antibody has been used in western blotting and immunostaining.
Monoclonal Anti-Spectrin (α and β) antibody produced in mouse has been used in flow cytometry analysis and confocal microscopy.

Biochem./physiol. Wirkung

Spectrins are multifunctional proteins, that modulates the cell morphology and mechanical properties. Spectrins also participates in the cell cycle by controlling the upregulation of membrane receptors. α- and β-spectrins are essential for the development of nervous system.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Membrane protein carbonylation in non-leukodepleted CPDA-preserved red blood cells
Kriebardis AG, et al.
Blood Cells, Molecules and Diseases, 36(2), 279-282 (2006)
Indicators of erythrocyte damage after microwave warming of packed red blood cells
Hirsch J, et al.
Clinical Chemistry, 49(5), 792-799 (2003)
Jan Hirsch et al.
Clinical chemistry, 49(5), 792-799 (2003-04-24)
Localized overheating of packed red blood cells (PRBCs) after microwave warming with consequent damage to erythrocytes has been reported. We therefore compared possible cellular markers of erythrocyte damage, as measured by flow cytometry, with laboratory indicators of hemolysis to evaluate
Kurt L Barkalow et al.
The Journal of cell biology, 161(3), 557-570 (2003-05-14)
Aspectrin-based skeleton uniformly underlies and supports the plasma membrane of the resting platelet, but remodels and centralizes in the activated platelet. alpha-Adducin, a phosphoprotein that forms a ternary complex with F-actin and spectrin, is dephosphorylated and mostly bound to spectrin
Spectrin labeling during oogenesis in zebrafish (Danio rerio)
Okuthe GE and Fabian BC
Acta Histochemica, 114(2), 177-181 (2012)

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