MAB1622

Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6

clone AA6, Chemicon^®, from mouse

• Synonym(e): (ALPHA)II-SPECTRIN, Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha chain, non-erythrocytic spectrin alpha, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Fodrin α
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: AA6, monoclonal
• Speziesreaktivität: pig, guinea pig, rabbit, mouse, chicken, rat, human
• Speziesreaktivität (Voraussage durch Homologie): mammals
• Hersteller/Markenname: Chemicon^®
• Methode(n): ChIP: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG1
• Eignung: not suitable for immunohistochemistry (Paraffin)
• NCBI-Hinterlegungsnummer: NM_003127.2
• UniProt-Hinterlegungsnummer: Q13813
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SPTAN1(6709)

Beschreibung

Allgemeine Beschreibung

Non-erythroid Spectrin, also known as Fodrin, plays a role in stabilizing membrane structures, maintaining cell shape and linking the cytoskeleton to plasma membranes or intracellular vesicles. As one of the primary targets cleaved by activated caspases during apoptosis, the full-length ~240 kDa protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa.

Spezifität

Specific for the 240, 150, and 120 kDa forms of alpha-spectrin/alpha-fodrin molecule of all mammalian nonerythroid cells and chicken alpha-spectrin.

Wide-mammalian reactivity is expected.

Immunogen

Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation.

Anwendung

Research Category
Zellstruktur

Research Sub Category
Cytoskelett

Immunohistochemistry (IFA):
A 1:200-1:400 dilution of a previous lot was used in immunohistochemistry.
Does not work on paraffin sections.

Optimal working dilutions must be determined by end user.

Chromatin Immunopreciptiation:
A previous lot of this antibody was used to immunoprecipitate Spectrin Alpha from chromatin-associated protein extracts derived from normal human, HeLa, FA-A, and transduced FA-A cells (McMahon, L.W., et al. (1999). J. Biological Chem. 274:32904-32908).

Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6 is an antibody against Spectrin alpha chain (nonerythroid) for use in IH, IP, WB, ChIP.

Qualität

Evaluated by Western Blot on staurosporine-treated HeLa lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected N-, C- terminal Spectrin alpha/Fodrin alpha on 10 μg of staurosporine treated HeLa lysates.

Zielbeschreibung

~240 kDa full length product, ~150 kDa N-terminal cleaved product and ~120 kDa C-terminal product may be seen in varing amounts in lysates, depending on condition. The smaller sizes represent break down products seen during apoptosis.

Physikalische Form

Protein A purified

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.09% sodium azide.

Lagerung und Haltbarkeit

Stable for 12 months at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
HeLa cells treated with staurosporine

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable