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Merck

RAB0431

Sigma-Aldrich

Mouse Shh-N ELISA Kit

for serum, plasma and cell culture supernatant

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About This Item

UNSPSC-Code:
41116158
NACRES:
NA.32

Speziesreaktivität

mouse

Verpackung

kit of 96 wells (12 strips x 8 wells)

Methode(n)

ELISA: suitable
capture ELISA: suitable

Aufnahme

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 4.1-1000 pg/mL

Nachweisverfahren

colorimetric

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

mouse ... Shh(20423)

Allgemeine Beschreibung

The Mouse Shh-N (Sonic Hedgehog N Terminus) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Shh-N in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse Sonic Hedgehog (N-terminus)

Anwendung

For research use only. Not for use in diagnostic procedures.
Mouse Shh-N ELISA Kit has been used to measure serum sonic hedgehog (Shh) level.

Biochem./physiol. Wirkung

Sonic hedgehog (Shh) is a secreted glycoprotein involved in embryonic morphogenic development of several tissues. It has a role in fracture healing and regeneration. In the canonical pathway, the protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer. Shh plays a vital role in axon growth and guidance throughout the brain development. Shh stimulates neurite outgrowth by overcoming neurite growth inhibitory environment associated with glial scar.

Sonstige Hinweise

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDB

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDB

  • RABSTOP3ELISA Stop Solution (Item I)SDB

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDB

  • RABWASH420X Wash Buffer (Item B)SDB

Piktogramme

Corrosion

Signalwort

Warning

H-Sätze

P-Sätze

Gefahreneinstufungen

Met. Corr. 1

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Reduced lipoapoptosis, hedgehog pathway activation and fibrosis in caspase-2 deficient mice with non-alcoholic steatohepatitis.
Machado MV
Gut, 64(7), 1148-1157 (2015)
An epigenetic switch induced by Shh signalling regulates gene activation during development and medulloblastoma growth.
Shi X
Nature Communications, 5:5425-5:5425 (2014)
Sonic hedgehog stimulates neurite outgrowth in a mechanical stretch model of reactive-astrogliosis.
Berretta A
Scientific Reports, 6:21896-6:21896 (2016)
Lin Ding et al.
Cellular and molecular gastroenterology and hepatology, 11(5), 1251-1266 (2020-12-22)
Helicobacter pylori infection in humans typically begins with colonization of the gastric antrum. The initial Th1 response occasionally coincides with an increase in gastrin secretion. Subsequently, the gastritis segues to chronic atrophic gastritis, metaplasia, dysplasia and distal gastric cancer. Despite
Expression and Role of Sonic Hedgehog in the Process of Fracture Healing with Aging.
Matsumoto K
In Vivo, 30(2), 99-105 (2016)

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