R7876
Ribonukleinsäure, Transfer aus Weizenkeimen
Type V, 15-19 units/mg solid
Synonym(e):
Transfer-RNA, tRNA
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Alle Fotos(3)
About This Item
Empfohlene Produkte
Qualität
for molecular biology
Qualitätsniveau
Typ
Type V
Form
lyophilized powder
Spezifische Aktivität
15-19 units/mg solid
Lagerbedingungen
desiccated
Löslichkeit
H2O: ~2 mg/mL, clear to hazy
Lagertemp.
2-8°C
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Allgemeine Beschreibung
Wheat germ tRNA contains many different specific tRNA molecules.
Anwendung
Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.
Komponenten
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Einheitendefinition
One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).
Ersetzt durch
Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Nature genetics, 30(3), 329-334 (2002-02-19)
Post-translational modification of histone tails is thought to modulate higher-order chromatin structure. Combinations of modifications including acetylation, phosphorylation and methylation have been proposed to provide marks recognized by specific proteins. This is exemplified, in both mammalian cells and fission yeast
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Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9716-9721 (2013-05-01)
After four decades of research aimed at understanding tRNA selection on the ribosome, the mechanism by which ribosomal ambiguity (ram) mutations promote miscoding remains unclear. Here, we present two X-ray crystal structures of the Thermus thermophilus 70S ribosome containing 16S
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