PURE1A
Protein A Antibody Purification Kit
sufficient for 10 purifications
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
12352203
NACRES:
NA.32
Empfohlene Produkte
Verwendung
sufficient for 10 purifications
Versandbedingung
wet ice
Lagertemp.
2-8°C
Allgemeine Beschreibung
The Protein A Antibody Purification Kit provides all of the reagents necessary to isolate monoclonal and polyclonal antibodies.
Spezifität
Capacity/Run (by Species)
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg
Anwendung
PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.
Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.
The Protein A Antibody Purification Kit is suitable for use with IgGs from human, mouse, rabbit, goat, and bovine species.
Leistungsmerkmale und Vorteile
- High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run
- Specific - will only bind IgG
- Easy to use - antibody is eluted and desalted in a single step, ready to use
- Gentle - avoids prolonged exposure of the antibody to low pH
Prinzip
Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.
Haftungsausschluss
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Binding Buffer 225 mL
- Desalting Cartridge 1 ea
- Elution Buffer 75 mL
- HEPES Buffer 225 mL
- 5 ml Syringe 1
- 10 ml Syringe 1
- Protein A Cartridge 1 mL
- Regeneration Buffer 75 mL
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Ähnliches Produkt
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Acute Tox. 4 Oral - STOT SE 1
Lagerklassenschlüssel
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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