PLA0227

Rabbit anti-BRD4 Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

• Synonym(e): CAP, HUNK1, HUNKI, MCAP, Protein HUNK1, bromodomain-containing 4, chromosome-associated protein
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Qualität: Powered by Bethyl Laboratories, Inc.
• Speziesreaktivität: human, mouse
• Konzentration: 1 mg/mL
• Methode(n): immunohistochemistry: 1:1,000-1:5,000; immunoprecipitation (IP): 2-5 μg/mg; western blot: 1:2,000-1:10,000
• Hinterlegungsnummer: NP_490597.1
• UniProt-Hinterlegungsnummer: O60885
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: rabbit ... BRD4(23476)

Beschreibung

Allgemeine Beschreibung

Bromodomain-containing protein 4 (BRD4) belongs to the bromodomain and extra-terminal (BET) family. The protein has two bromodomains (BD1 and BD2) connected by a long interdomain linker.

Immunogen

The epitope recognized by PLA0227 maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476).

Biochem./physiol. Wirkung

Bromodomain-containing protein 4 (BRD4) works as a reader of epigenetic marks and is involved in chromatin remodeling and transcriptional regulation. Therefore, it is a target for oncology, inflammation and cardiovascular disease. This gene is upregulated in hepatocellular carcinoma and is associated with poor prognosis. BRD4 is also associated with melanoma, hepatocellular carcinoma, multiple myeloma, Burkitt′s lymphoma, acute myeloid leukemia and breast cancer. BRD4 connects cell cycle and transcription, works as a scaffold for transcription factors and bookmarks active genes during mitosis. It interacts with acetylated histone-3 and histone-4 tails and helps in maintaining chromatin architecture. Also, it regulates transcription elongation via phosphorylation of RNA polymerase II.

Physikalische Form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Sonstige Hinweise

Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable