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P3026

Sigma-Aldrich

Pektolyase aus Aspergillus japonicus

greener alternative

lyophilized powder, ≥0.3 units/mg solid

Synonym(e):

Pectolytic enzyme

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About This Item

EC-Nummer:
3.2.1.15 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00131813
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

Aspergillus sp. (A. japonicus)

Form

lyophilized powder

Spezifische Aktivität

≥0.3 units/mg solid

Mol-Gew.

50 kDa

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Grünere Alternativprodukt-Kategorie

, Enabling

Lagertemp.

2-8°C

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Allgemeine Beschreibung

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Pectolyase or pectin lyase is stable in the temperature range of 40-50°C and is a 50 kDa enzyme with an isoelectric pH of 3.8.

Anwendung

Pectolyase from Aspergillus japonicus has been used:
  • in the lysis of chlorella cells for protoplast generation
  • as component of enzyme digestion buffer for Arabidopsis meiotic chromosome isolation
  • to induce reorganization of Arabidopsis root cap cell architecture

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. It is used in complex carbohydrate analysis. Product P3026 is provided in a lyophilized powder containing lactose. Product P3026 has been used to digest Crassula root tips prior to meiotic analysis.
Zur Herstellung von Pflanzenprotoplast für den Zellwandabbau vor der organellen Isolation.

Biochem./physiol. Wirkung

Pectolyase catalyzes the eliminative cleavage of a-(1- 4)-Dgalacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-a-D-galact-4-enuronosyl groups at their non-reducing ends. It contain two types of pectinase, endopolygalacturonase, endo-pectin lyase and a maceration stimulating factora.
Pectolyase is used for protoplast isolation from plant cells. It also mediates ethylene hormone synthesis.

Sonstige Hinweise

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.
Für weitere Informationen siehe enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Einheitendefinition

1 U setzt 1.0 μmol Galacturonsäure aus Polygalacturonsäure pro Minute frei bei pH 5.5 und 25 °C.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

P J De Lange et al.
Annals of botany, 101(6), 881-899 (2007-12-07)
Crassula hunua and C. ruamahanga have been taxonomically controversial. Here their distinctiveness is assessed so that their taxonomic and conservation status can be clarified. Populations of these two species were analysed using morphological, chromosomal and DNA sequence data. It proved
Identification of sporopollenin as the outer layer of cell wall in microalga Chlorella protothecoides
He X, et al.
Frontiers in Microbiology, 7, 1047-1047 (2016)
Sam J Parkinson et al.
Nature communications, 13(1), 7385-7385 (2022-12-01)
As agriculture strives to feed an ever-increasing number of people, it must also adapt to increasing exposure to minute plastic particles. To learn about the accumulation of nanoplastics by plants, we prepared well-defined block copolymer nanoparticles by aqueous dispersion polymerisation.
Susann Hesse et al.
Frontiers in plant science, 10, 773-773 (2019-07-10)
During prophase I a meiosis-specific proteinaceous tripartite structure, the synaptonemal complex (SC), forms a scaffold to connect homologous chromosomes along their lengths. This process, called synapsis, is required in most organisms to promote recombination between homologs facilitating genetic variability and
Protocol for the preparation of Arabidopsis meiotic chromosome spreads and fluorescent in situ hybridization
Bolanos-Villegas P, et al.
The Plant Journal, 4, e1102-e1102 (2014)
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Artikel

Carbohydrate Analysis

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