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P1261
Phosphorylase a from rabbit muscle
lyophilized powder, 20-30 units/mg protein
Synonym(e):
1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase
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About This Item
Empfohlene Produkte
Biologische Quelle
rabbit muscle
Qualitätsniveau
Form
lyophilized powder
Spezifische Aktivität
20-30 units/mg protein
Aufgereinigt durch
2× crystallization
Zusammensetzung
Protein, 30-60% E1%/280
Fremdaktivität
AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%
phosphoglucomutase ≤0.25%
phosphorylase b ≤30%
Lagertemp.
−20°C
Verwandte Kategorien
Allgemeine Beschreibung
Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P).
Anwendung
Phosphorylase a from rabbit muscle has been used to mix with glycogen for the preparation of limit dextrin. It has also been used to probe the array in oligosaccharide microarray analysis of phosphorylase activity.
Phosphorylase from rabbit muscle has been used in a study to assess the molecular mechanisms of oleanolic acid. It has also been used in a study to describe the conversion of phosphorylase B to A, through a conversion enzyme in the presence of 32P-ATP.
Biochem./physiol. Wirkung
Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.
Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls.
Phosphorylase participates in glycogenolysis. Deficiency of phosphorylase leads to several glycogen storage diseases with hepatomegaly.
Einheitendefinition
One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30 °C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)
Physikalische Form
Lyophilized powder containing β-glycerophosphate and EDTA
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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