N9284
Nitroreductase from Escherichia coli
≥90% (SDS-PAGE), recombinant, expressed in E. coli
Synonym(e):
NTRA
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Rekombinant
expressed in E. coli
Qualitätsniveau
Assay
≥90% (SDS-PAGE)
Form
lyophilized powder
Spezifische Aktivität
≥100 units/mL
Mol-Gew.
monomer 24000
Grünere Alternativprodukt-Eigenschaften
Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
UniProt-Hinterlegungsnummer
Grünere Alternativprodukt-Kategorie
Versandbedingung
wet ice
Lagertemp.
−20°C
Angaben zum Gen
Escherichia coli K12 ... nfsB(945483)
Allgemeine Beschreibung
Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.
Anwendung
Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.
Biochem./physiol. Wirkung
Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.
Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.
Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.
Physikalische Eigenschaften
Lyophilized powder containing PBS. Does not contain BSA as excipient
Einheitendefinition
One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.
Angaben zur Herstellung
Produced using animal component-free materials.
Lagerklassenschlüssel
13 - Non Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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