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MWGF70

Sigma-Aldrich

Gel Filtration Markers Kit for Protein Molecular Weights 6,500-66,000 Da

Synonym(e):

Gel Filtration Protein Ladder

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.25

Form

solid

Qualitätsniveau

200

Methode(n)

protein quantification: suitable

Lagertemp.

−20°C

Allgemeine Beschreibung

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins. Fractionation is based on the diffusion of molecules into the pores of the resin. Larger proteins do not enter the pores of the resin as readily but pass through the fluid volume of the column faster than smaller proteins. These protein molecules elute from the column in order of decreasing molecular mass.

Anwendung

Gel Filtration Markers Kit for Protein Molecular Weights 6,500-66,000 Da has been used as a standard in size exclusion chromatography.
Gel filtration markers are used in protein chromatography and gel filtration chromatography. Gel filtration markers have been used to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system. Gel filtration markers have also been used to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner.

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB
  • A8531Albumin, Bovine serum, 66,000 MW 1 mL/vialSDB
  • A3886Aprotinin, Bovine lung, 6,500 MW 1 mL/vialSDB
  • D4772Blue Dextran 2,000,000 MW 1 mL/vialSDB
  • C7025Carbonic Anhydrase, Bovine erythrocytes, 29,000 MW 1 mL/vialSDB
  • C7150Cytochrome c, Horse heart, 12,400 MW 1 mL/vialSDB

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Analysenzertifikate (COA)

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Filomena D'Amato et al.
The Journal of endocrinology, 197(2), 359-369 (2008-04-25)
While vg f gene knockout mice are hyperactive and hypermetabolic, surprisingly the TLQP-21 brain VGF peptide increased energy consumption, suggesting that opposing regulatory effects could be exerted by peptides alternatively cleaved from the VGF precursor. Using antisera to the VGF
N Heredia et al.
Letters in applied microbiology, 48(1), 133-139 (2008-12-06)
The mechanisms of adaptation of Clostridium perfringens to high temperatures are not well understood. In this work, the involvement of extracellular compounds in protection to heat was determined. Cells were grown in fluid thioglycollate medium or chicken broth. When mid-log
Francesca Marcon et al.
Toxicon : official journal of the International Society on Toxinology, 58(5), 439-452 (2011-08-23)
The venom of the Australian lowlands copperhead, Austrelaps superbus, produces significant and potentially lethal neurotoxic paralysis in cases of clinical envenomation. However, little is known about the neurotoxic components within this venom or venoms from the related alpine copperhead (Austrelaps
Barbara Noli et al.
PloS one, 10(11), e0141193-e0141193 (2015-11-12)
VGF mRNA is induced in specific hypothalamic areas of the Siberian hamster upon exposure to short photoperiods, which is associated with a seasonal decrease in appetite and weight loss. Processing of VGF generates multiple bioactive peptides, so the objective of
Cristina Giatti Marques De Souza et al.
Journal of basic microbiology, 43(4), 278-286 (2003-07-23)
The wood-degrading fungus Pleurotus pulmonarius produces at least two laccase isoforms, Lcc1 and Lcc2, when grown on wheat bran solid state medium. The main laccase, Lcc2, was purified to apparent electrophoretic homogeneity by using acetone precipitation, anion-exchange chromatography and gel
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