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Sigma-Aldrich

MISSION® Target ID Library

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About This Item

UNSPSC-Code:
41105904
NACRES:
NA.51

Verpackung

pkg of 100 μL (library)
pkg of 200 μL (primer 1)
pkg of 200 μL (primer 2)

Konzentration

1 ng/μL (library)
25 μM (primer 1)
25 μM (primer 2)

Versandbedingung

wet ice

Lagertemp.

−20°C

Allgemeine Beschreibung

The Target ID Library enables bench-top transcriptome-wide human miRNA and ncRNA gene target identification. It has an innovative dual selection system, which makes rapid whole transcriptome miRNA and ncRNA gene target screens accessible to any researcher with minimal reagent, time, or capital equipment expense.

The Target ID Library is a transcriptome wide cDNA pool produced using a proprietary process. Each library is tested for gene representation to ensure robust library coverage. The library is provided in ready-to-use DNA format, with enough material for multiple genome-wide screens.

The Target ID Library is a pool of plasmids, each with a human cDNA inserted into the 3′-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing

Download deep sequencing data of target ID library.

Komponenten

Library: 100μl (approx 1μg/μl), frozen liquid, store at -10°C to -25°C.
Primer 1: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Primer 2: 200μl (25μM), frozen liquid, store at -10°C to -25°C

Rechtliche Hinweise

MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Erforderlich, aber nicht bereitgestellt

Produkt-Nr.
Beschreibung
Preisangaben

G2536

Ganciclovir, ≥99% (HPLC), powder

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Joop Gäken et al.
Nucleic acids research, 40(10), e75-e75 (2012-02-11)
MicroRNAs (miRNA) are a class of small RNA molecules that regulate numerous critical cellular processes and bind to partially complementary sequences resulting in down-regulation of their target genes. Due to the incomplete homology of the miRNA to its target site
Shok Ping Lim et al.
Journal of leukocyte biology, 103(5), 855-866 (2018-04-16)
MicroRNAs are short endogenous noncoding RNAs that play pivotal roles in a diverse range of cellular processes. The miR-181 family is important in T cell development, proliferation, and activation. In this study, we have identified BRK1 as a potential target

Artikel

MISSION® Target ID Library for Human miRNA Target ID and Discovery

MISSION® Target ID Library for Human miRNA Target Identification and Discovery;

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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