MAK118
Invertase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(e):
β-fructofuranosidase Assay Kit
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About This Item
UNSPSC-Code:
12161503
NACRES:
NA.84
Empfohlene Produkte
Verwendung
sufficient for 100 colorimetric or fluorometric tests
Anwendung(en)
cosmetics
environmental
food and beverages
Nachweisverfahren
colorimetric
fluorometric
Lagertemp.
−20°C
Allgemeine Beschreibung
Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.
Anwendung
Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.
Leistungsmerkmale und Vorteile
Compatible with high-throughput handling systems.
Eignung
Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.
Prinzip
In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.
Lagerklassenschlüssel
10 - Combustible liquids
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Identification and Functional Characterization of Sugarcane Invertase Inhibitor (ShINH1): A Potential Candidate for Reducing Pre-and Post-harvest Loss of Sucrose in Sugarcane.
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Journal of experimental botany, 67(11), 3303-3312 (2016-04-17)
Invertases are a widespread group of enzymes that catalyse the conversion of sucrose into fructose and glucose. Plants invertases and their substrates are essential factors that play an active role in primary metabolism and in cellular differentiation and by these
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In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play
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