MAK095
Aspartate Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
UNSPSC-Code:
12161503
NACRES:
NA.84
Empfohlene Produkte
Verwendung
sufficient for 100 colorimetric or fluorometric tests
Nachweisverfahren
colorimetric
fluorometric
Relevante Krankheit(en)
endocrinological disorders, diabetes; neurological disorders
Lagertemp.
−20°C
Allgemeine Beschreibung
Aspartate, the carboxylate anion of aspartic acid, is an acidic, non-essential amino acid involved in protein synthesis and multiple other cellular biochemical pathways. Aspartate contributes to nucleotide synthesis via the synthesis of the precursor inosine mono-phosphate. In the urea cycle, aspartate is a key metabolite, donating a nitrogen group towards the formation of urea. Aspartate is also critical for oxidative phosphorylation as part of the aspartate-malate shuttle, which transfers reducing equivalents across the mitochondrial membrane.
Anwendung
Aspartate Assay Kit has been used to measure the aspartate levels in nerve cells and Lactococcus lactis strain cultured in lysogeny broth (LB) medium.
Eignung
The Aspartate Assay Kit is suitable for aspartate detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.
Prinzip
Aspartate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the aspartate present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).
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Produkt-Nr.
Beschreibung
Preisangaben
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
188.6 °F - closed cup
Flammpunkt (°C)
87 °C - closed cup
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