MAK082

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit

sufficient for 100 colorimetric tests

• Synonym(e): ALDH Activity Assay Kit
• UNSPSC-Code: 12161503
• NACRES: NA.84

Eigenschaften

• Verwendung: sufficient for 100 colorimetric tests
• Nachweisverfahren: colorimetric
• Relevante Krankheit(en): cardiovascular diseases; cancer; hematological disorder
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Aldehyde dehydrogenases (ALDH) are a family of enzymes that catalyze the metabolism of exogenous and endogenous aldehydes, preventing the accumulation of potentially reactive and toxic aldehydes and their metabolites. In addition to their role in aldehyde metabolism, ALDH enzymes also play critical roles in other cellular processes such as cell proliferation, differentiation, and survival. Mutations in ALDH family members have been linked to numerous diseases such as heart disease, cataracts, and certain cancers. ALDH activity may also serve as a marker for certain stem cell populations including hematopoietic stem cells and certain cancer stem cells.

Anwendung

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the activity of aldehyde dehydrogenase.

Leistungsmerkmale und Vorteile

Compatible with high-throughput handling systems.

Eignung

Suitable for the measurement of aldehyde dehydrogenase (ALDH) in a variety of samples including tissue and cells

Prinzip

The Aldehyde Dehydrogenase Activity Colorimetric Assay kit provides a simple and direct procedure for measuring NAD-dependent ALDH activity in a variety of samples. ALDH activity is determined by a coupled enzyme assay in which acetaldehyde is oxidized by ALDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the ALDH activity present. One unit of ALDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.

Sicherheitshinweise

• Piktogramme: GHS08,GHS05
• Signalwort: Danger
• H-Sätze: H314,H350
• P-Sätze: P202 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Gefahreneinstufungen: Carc. 1B - Eye Dam. 1 - Skin Corr. 1B
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects