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Merck

M4892

SAFC

McCoy 5A-Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Arzneimittelherstellung

Synonym(e):

5A basal medium

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352207
NACRES:
NA.71

Qualitätsniveau

Form

powder

Methode(n)

cell culture | mammalian: suitable

Komponenten

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Anwendung

McCoy′s 5A Medium has been used for culturing:
  • human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
  • human colorectal HCT116 cells to study miR-224 over-expression in cancer
  • osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Biochem./physiol. Wirkung

McCoy′s 5A-Medium wurde ursprünglich zur Unterstützung von Lebertumorzellen durch die Modifikation der in BME vorkommenden Aminosäuren entwickelt. Diese Formulierung wird auch zur Unterstützung des Wachstums in Primärkulturen von Knochenmark, Haut, Gingiva, Niere, Omentum, Nebenniere, Lunge, Milz, Rattenembryos und anderen Zelltypen verwendet.

Menge

Formulated to contain 11.9 grams of powder per liter of medium.

Rekonstituierung

Supplement with 2.2 g/L sodium bicarbonate.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3


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Yu Wang et al.
PloS one, 8(7), e68744-e68744 (2013-08-08)
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