L3272

Luria Agar Basis (Miller Modifikation)

Powder microbial growth medium

• Synonym(e): Luria Agar Base (Miller’s Modification)
• UNSPSC-Code: 41106200
• NACRES: NA.85

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 200
• Sterilität: non-sterile
• Form: powder
• Zusammensetzung: Agar, 15 g/L ; NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• Methode(n): microbiological culture: suitable
• pH-Wert: 6.8-7.2(3.05% solution)
• Anwendung(en): food and beverages; microbiology
• Lagertemp.: room temp
• Eignung: nonselective for Escherichia coli; nonselective for coliforms

Beschreibung

Allgemeine Beschreibung

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

Anwendung

Für die Erhaltung und Vermehrung von Escherichia coli.

LB Broth with agar (Luria low salt) has been used:

• for culturing E. coli DH10B cells.
• in the isolation of diazotrophic bacteria.
• for transformation of competent E. coli strain Stbl3 for cloning.

Leistungsmerkmale und Vorteile

Luria′s low salt LB powder with agar provides:

• Easy scale-up using larger package sizes
• A budget-friendly alternative to pre-poured plates
• Standard formulation

Angaben zur Herstellung

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

Rechtliche Hinweise

Zeocin is a registered trademark of Cayla Sarl

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable