L2650

Lektin aus Glycine max (Sojabohne)

peroxidase conjugate, lyophilized powder

• Synonym(e): Sojabohnen Agglutinin
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: Glycine max (soybean)
• Konjugat: peroxidase conjugate
• Assay: 85.00-100.00% (Warburg-Christian)
• Form: lyophilized powder
• Wirksamkeit: <80 μg per mL agglutination activity
• peroxidase activity: 25-100 units/mg protein
• Zusammensetzung: Protein, ~95% modified Warburg-Christian
• Methode(n): western blot: suitable
• UniProt-Hinterlegungsnummer: P05046
• Lagertemp.: −20°C
• Angaben zum Gen: soybean ... LE1(100818710)

Beschreibung

Anwendung

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

Biochem./physiol. Wirkung

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

Einheitendefinition

1 U bildet 1 mg Purpurogallin in 20 Sek. aus Pyrogallol bei pH 6.0 und 20 °C.

Physikalische Form

Enthält Natriumcitrat

Angaben zur Herstellung

Conjugates are prepared from affinity purified lectin.

Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Hinweis zur Analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Sicherheitshinweise

• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)