H9789

Anti-HMGB2/HMG2 in Kaninchen hergestellte Antikörper

affinity isolated antibody

• Synonym(e): Anti-High mobility group box 2, Anti-High mobility group protein 2
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: rat, human, mouse
• Verpackung: antibody small pack of 25 μL
• Methode(n): immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates.; indirect immunofluorescence: 1-2 μg/mL using fixed PC12 cultured cells.; western blot: 2-4 μg/mL using PC12 whole cell lysates.
• UniProt-Hinterlegungsnummer: P26583
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... HMGB2(3148) ; mouse ... Hmgb2(97165) ; rat ... Hmgb2(29395)

Beschreibung

Allgemeine Beschreibung

The high mobility group (HMG) proteins are subdivided into 3 families based on their functional sequence motif characteristic: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) and the HMGA (formerly HMG-I/Y/C) family. The HMGB protein family, including HMGB1, HMGB2, and HMGB3, are highly conserved (more than 80% amino acid identity), and indistinguishable in their biochemical properties. The high-mobility group box 2 (HMGB2) is a ~25 kDa protein of 209 amino acids that consists of two homologous HMG-boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMGB2 is restricted mainly to lymphoid organs and testes, although it is widely expressed in embryogenesis.

Immunogen

synthetic peptide corresponding to amino acid 165-181 of human HMGB2, conjugated to KLH. The corresponding sequence is conserved in mouse and differs by one amino acid in rat.

Anwendung

Anti-HMGB2 (HMG2) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol.

Biochem./physiol. Wirkung

The high-mobility group box 1 and 2 (HMGB 1 & 2) proteins participate in the regulation of chromatin structure as well as being involved in transcription regulation, DNA repair, recombination, differentiation and extracellular signalling.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)