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GE29-0005-94

His GraviTrap TALON®

Cytiva 29-0005-94, pack of 10 columns

Synonym(e):

His GraviTrap

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

Verpackung

pack of 10 columns

Hersteller/Markenname

Cytiva 29-0005-94

Bettvolumen

1 mL

Säule ID

15 mm

Matrix

6% cross-linked agarose

Partikelgröße

60-160 μm

cleaning

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

Kapazität

≤15 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

Lagertemp.

2-8°C

Allgemeine Beschreibung

His GraviTrap Talon® is a prepacked gravity f low column for convenient manual purification of histidine-tagged proteins.

Anwendung

His GraviTrap Talon® gravity flow columns are designed for convenient and efficient gravity flow purification of histidine-tagged Proteins directly from clarified or unclarified cell lysates. His GraviTrap Talon® columns are delivered in a package that can be converted into a column stand to simplify purification, eliminating the need for additional equipment to complete the protocol. Special column frits protect the chromatography medium from running dry during purification.

Large sample volumes can be applied in one go, and the histidine-tagged Protein is effectively eluted in a small volume. LabMate PD-10 Buffer Reservoir can be connected to the columns for handling of sample volumes above 10 ml. Leakage of cobalt ions from Talon® Superflow medium is negligible.

Leistungsmerkmale und Vorteile

  • Convenient histidine-tagged purification with high purity (>90%) and binding capacity of up to 15 mg/column.
  • Ready-to-use gravity flow column prepacked with 1 mL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
  • One run takes about 30 minutes, and large sample volumes can be applied all at once.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

GraviTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

This page describes manual purification of histidine-tagged proteins using His GraviTrap TALON from GE Healthcare.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Protokolle

This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.

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