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GE28-9321-71

HIS Spintrap™ Kit

Cytiva 28-9321-71, pack of 1 kit

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.56

Verpackung

pack of 1 kit

Hersteller/Markenname

Cytiva 28-9321-71

Lagerbedingungen

(0.15% Kathon CG)
 (20% Ethanol)

Matrix

highly cross-linked 6% agarose

Durchschnittliche Teilgröße

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

Kapazität

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Leistungsmerkmale und Vorteile

  • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
  • Ni Sepharose High Performance in prepacked spin columns.
  • Yields up to 750 µg pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

Komponenten

The kit contains:

1 pack - GE28-4013-53 - His Spin Trap™
1 pack - GE11-0034-00 - His Buffer Kit

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

SpinTrap is a trademark of Cytiva

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB
  • GE11-0034-00His Buffer Kit, Cytiva 11-0034-00SDB
  • GE28-4013-53His Spintrap™, Cytiva 28-4013-53SDB

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H314

Lagerklassenschlüssel

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Optimizing Purification of Histidine-Tagged Proteins

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using GE Healthcare products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Protokolle

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from GE Healthcare.

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.

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