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GE17-5282-02

GSTrap High Performance

Cytiva 17-5282-02, pack of 5 × 5 mL

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About This Item

UNSPSC-Code:
23151817
NACRES:
NA.56

ligand

glutathione

Verpackung

pack of 5 × 5 mL

Hersteller/Markenname

Cytiva 17-5282-02

Parameter

<20 mL/min flow rate
42 psi (H2O at room temperature.)

Bettgröße

16 mm × 25 mm

Bettvolumen

5 mL

Säule ID

16 mm

Matrix

highly cross-linked 6% agarose

Durchschnittliche Teilgröße

34 μm

cleaning

3-12

working range

3-12

Kapazität

>7 mg/mL binding capacity (medium)

Allgemeine Beschreibung

GSTrap HP columns are prepacked with Glutathione Sepharose High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.

GSTrap HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.

GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Leistungsmerkmale und Vorteile

  • High resolution purification of GST-tagged proteins.
  • Mild elution conditions preserving protein antigenicity and function.
  • Fast, one-step purification.
  • Easy to use with syringe, pump or a chromatography system.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

Alle anzeigen

Protokolle

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

Characteristics of Glutathione Sepharose® Products

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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