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Merck

GE17-5068-01

Sigma-Aldrich

SOURCE 15RPC ST 4.6/100

pkg of 1 column

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About This Item

UNSPSC-Code:
41115700
NACRES:
SB.52

ligand

matrix surface

Materialien

polypropylene column

Leistungsmerkmale

autoclavable 20 min at 121°C

Verpackung

pkg of 1 column

Parameter

flow rate
0.5 ml/min - 2.5 ml/min flow rate
580 psi

Methode(n)

HPLC: suitable

Bettgröße

4.6 mm × 100 mm

Bettvolumen

1.7 mL

Säule ID

4.6 mm

Matrix

polymeric
polystyrene/divinylbenzene

Aktive Matrixgruppe

polystyrene-divinylbenzene phase

Durchschnittliche Teilgröße

15 μm

cleaning

1-14

working range

1-12

Kapazität

~10 mg binding capacity

Eignung

suitable for bioprocess medium

Trenntechnik

reversed phase

Allgemeine Beschreibung

SOURCE 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h

Anwendung

SOURCE 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE 15RPC offers properties superior to those of other polymeric matrices.

Leistungsmerkmale und Vorteile

  • Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
  • Excellent scalability, from RESOURCE to FineLINE columns.
  • using ÄKTA® design and other high-performance liquid chromatography systems.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

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Rechtliche Hinweise

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

This page discusses column packing and preparation techniques for reverse phase chromatography

Protokolle

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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