GALS

β-Galactosidase Reporter Gene Staining Kit

• Synonym(e): β-galactosidase, LacZ
• UNSPSC-Code: 12161503
• NACRES: NA.32

Eigenschaften

• Verwendung: kit sufficient for 100 tests (using a 3.5 cm dish)
• Qualitätsniveau: 200
• Methode(n): microbe id | staining: suitable
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.

Anwendung

β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:

• sagittal brain sections in histoenzymology
• pancreas or colon sections in β-galactosidase reporter gene assay
• mice E10.5 -11.5 embryo and embryo sections

Sonstige Hinweise

The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.

Sicherheitshinweise

• Piktogramme: GHS02,GHS06,GHS08,GHS05
• Signalwort: Danger
• H-Sätze: H226,H302 + H312,H314,H317,H331,H334,H335,H341,H350,H412
• P-Sätze: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Zielorgane: Respiratory system
• Zusätzliche Gefahrenhinweise: EUH032
• Lagerklassenschlüssel: 3 - Flammable liquids
• Flammpunkt (°F): 132.8 °F
• Flammpunkt (°C): 56 °C