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G9880

Sigma-Aldrich

Glutam-Brenztraubentransaminase aus Schweineherz

ammonium sulfate suspension, ≥75 units/mg protein

Synonym(e):

L-Alanine: 2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT

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About This Item

CAS-Nummer:
9000-86-6
EC-Nummer:
2.6.1.2 (BRENDA, IUBMB)
Beilstein:
3562140
EG-Nummer:
232-561-4
MDL-Nummer:
MFCD00131192
UNSPSC-Code:
12352204
NACRES:
NA.54

Biologische Quelle

Porcine heart

Form

ammonium sulfate suspension

Spezifische Aktivität

≥75 units/mg protein

Mol-Gew.

42 kDa

Fremdaktivität

L-glutamic dehydrogenase, lactic dehydrogenase and malic dehydrogenase ≤0.01%
glutamic-oxalacetic transaminase ≤0.05%

Versandbedingung

wet ice

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Research area: Cell Signaling
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body but is found in highest concentrations in the liver. Two GPT isoforms, GPT1 and GPT2, are present in mammals.

Anwendung

Glutamic-Pyruvic Transaminase from porcine heart has been used to determine L-lactate levels in aqueous polyethylene glycol (PEG) solutions.

Biochem./physiol. Wirkung

GPT is responsible for catalyzing the reversible transamination of α-ketoglutarate and alanine to glutamate and pyruvate. It also plays a critical role in the metabolism of amino acids and gluconeogenesis.
Glutamic-pyruvic transaminase (GPT) is located in most cells in the body, but is found in highest concentrations in the liver. It is used as a marker of liver disease due to the release of GPT into the blood following liver cell necrosis.

Einheitendefinition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.6 at 37 °C in the presence of L-alanine.

Physikalische Form

Suspension in 1.8 M (NH4)2SO4 solution, pH 6.0

Hinweis zur Analyse

Protein determined by biuret.

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

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Hepatitis B surface antigen (HBsAg) is regarded as sole marker of hepatitis B virus (HBV) infection in Bangladesh and most other developing countries. However, some HBV-negative subjects may harbor HBV DNA and transfusion of their blood may cause HBV infection
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