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G3153
ββ-Galactosidase aus E. coli
lyophilized powder, ≥500 units/mg protein
Synonym(e):
β-D-Galactosid-Galactohydrolase, Lactase
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About This Item
Empfohlene Produkte
Rekombinant
expressed in E. coli
Qualitätsniveau
Form
lyophilized powder
Spezifische Aktivität
≥500 units/mg protein
Mol-Gew.
465 kDa
Lagertemp.
−20°C
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Allgemeine Beschreibung
β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.
Anwendung
β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).
β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.
Biochem./physiol. Wirkung
β-Galactosidase spaltet Lactose in ihre Monosaccharid-Komponenten Glucose und Galactose. Außerdem katalysiert sie die Transglycosylierung von Glucose zu Allolactose, dem Induktor von β-Galactosidase, in einem Rückführungsmechanismus.
β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.
Verpackung
Package size based on protein content
Physikalische Eigenschaften
Molekulargewicht des Tetramers: 465 kDa (Subunits je 116,3 kDa)
Einheitendefinition
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Physikalische Form
Stabilized with phosphate buffer and sucrose
Inhibitor
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Substrat
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Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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