FG0445

Dulbecco′s Modified Eagle′s Medium - high glucose

With 4500 mg/L glucose, L-Ala-L-Gln, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• UNSPSC-Code: 12352207

Eigenschaften

• Qualitätsniveau: 400
• Sterilität: sterile-filtered; sterile
• Form: liquid
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• Komponenten: NaHCO₃: yes; stable glutamine: yes; glucose: high; phenol red: yes; HEPES: no; sodium pyruvate: yes
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Anwendung

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable