F7053

Monoklonaler Anti-Vinculin-Antikörper in Maus hergestellte Antikörper

clone hVIN-1, purified from hybridoma cell culture

• Synonym(e): Monoclonal Anti-Vinculin antibody produced in mouse
• MDL-Nummer: MFCD00162905
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: FITC conjugate
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: hVIN-1, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 116 kDa
• Speziesreaktivität: frog, chicken, mouse, canine, human, bovine, rat, turkey
• Lagerbedingungen: protect from light
• Methode(n): direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P18206
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

Beschreibung

Spezifität

Markiert spezifisch Vinculin an Zell-Zell- und Zell-Substrat-Kontakten. Reagiert stark mit humanem Vinculin. Zeigt Kreuzreaktivität mit Glattmuskel-Metavinculin.

Immunogen

Aufgereinigtes humanes Vinculin aus dem Uterus.

Anwendung

Monoclonal Anti-Vinculin-FITC antibody is suitable for immunofluorescence staining of fibroblasts cells to study the effects of nanofiber spatial organization on cell behaviors. It is also suitable for direct immunofluorescence at a dilution of 1:50 using methanol/acetone fixed cultured bovine MDBK cells.

Biochem./physiol. Wirkung

Vinculin is a cytoskeletal protein with molecular mass of ~116 kDa. Vinculin is closely associated with adhesive membrane areas, such as focal contacts, podosomes, or intercellular junctions of the zonula adherens type. It acts as a mediator in interactions of integrins and the actin cytoskeleton. It forms a bridge between integrins, receptors for ECM molecules on the cell surface and the F-actin cytoskeleton. It is associated with talin and α-actinin which directly connects with integrins. It has ability to bind α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable