E8656
Heat-Labile Enterotoxin, B subunit aus E. coli
recombinant, expressed in Pichia pastoris, >90% (SDS-PAGE), lyophilized powder
Synonym(e):
LTB
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About This Item
UNSPSC-Code:
12352200
NACRES:
NA.77
Empfohlene Produkte
Rekombinant
expressed in Pichia pastoris
Qualitätsniveau
Assay
>90% (SDS-PAGE)
Form
lyophilized powder
Versandbedingung
wet ice
Lagertemp.
2-8°C
Anwendung
Heat-Labile Enterotoxin, B subunit (LTB) from E. coli has been used in enzyme-linked immunosorbent assay (ELISA).
Biochem./physiol. Wirkung
Enterotoxigenic Escherichia coli causes diarrhea through its heat-labile enterotoxin (LT). The LT is a periplasmic protein composed of one A subunit (LTA, 27 kDa) and five non-covalently associated B subunits (LTB, 11.6 kDa each) forming a ring-like pentamer. LTB has high affinity towards the toxin receptor ganglioside GM1, a glycosphingolipid found ubiquitously on the surface of mammalian cells. Ganglioside GM1 facilitates the delivery of the A subunit to the cytosol of the target cell resulting in persistent synthesis of cAMP and subsequently diarrhea. This characteristic makes LTB a good label for microglial cells (due to the enrichment of ganglioside GM1 on their cell surface). In addition, studies made mostly with animal models demonstrated that recombinant LTB could stimulate strong serum and mucosal immune responses against LT. Other studies have indicated that LTB could be used as a potent mucosal adjuvant.
Leistungsmerkmale und Vorteile
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Physikalische Form
Lyophilized from a solution containing Heat-Labile Enterotoxin, B subunit in 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3, and 1 mM sodium EDTA.
Hinweis zur Analyse
The activity is measured by ELISA using ganglioside GM1-coated plates (Cat. No. G7641). LTB at various concentrations is incubated on the ganglioside GM1 coated wells, followed by anti-cholera toxin antibody (Cat. No. C3062), and peroxidase-labeled goat anti-rabbit IgG (Cat.No. A0545) as the secondary antibody. Binding saturation of 50% may be achieved with = 0.12 μg/mL of LTB.
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Acute Tox. 4 Dermal - Aquatic Chronic 3
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Analysenzertifikate (COA)
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