DUO96020

Duolink^® PLA Multicolor Probemaker Kit - Green

Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events

• Synonym(e): in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit
• UNSPSC-Code: 41105331
• NACRES: NA.32

Eigenschaften

• Produktlinie: Duolink^®
• Methode(n): immunofluorescence: suitable; multiplexing: suitable; proximity ligation assay: suitable
• Fluoreszenz: λ_ex 490 nm; λ_em 520 nm (FITC); λ_ex 490 nm; λ_em 520 nm (green)
• Eignung: suitable for fluorescence
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Anwendung

Specificity
Green fluorescence detection reagents are often used with FITC filter.

Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

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Leistungsmerkmale und Vorteile

Multicolor Duolink^® proximity ligation assay (PLA^®) is an application of the Duolink^® technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink^® PLA Probemaker Guide for Multicolor Detection and the Duolink^® PLA Multicolor Detection protocol to use this product.

• Visualize and detect multiple proteins events in one experiment.
• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Detection of low abundant proteins due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Faster and more simple than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Komponenten

Duolink^® PLA Multicolor Probemaker Kit - Green contains all the necessary reagents to perform the conjugations of Green Oligo C and GreenOligo D directly to primary antibodies. The resulting PLA probes (Green C and Green D) must be used together and cannot be paired with any other Duolink^® PLA Probe.

This product is comprised of the following:

• Green Oligo C DUO86020C
• Green Oligo D DUO86020D
• Conjugation Buffer DUO82033
• Stop Reagent DUO82034
• Storage Solution DUO82035

See datasheet for more information.

To perform a Multicolor Duolink^® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink^® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink^® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink^® Wash Buffers and Mounting Medium.

Rechtliche Hinweise

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS07,GHS09
• Signalwort: Warning
• H-Sätze: H317,H411
• P-Sätze: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Gefahreneinstufungen: Aquatic Chronic 2 - Skin Sens. 1
• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3