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DMN10

Sigma-Aldrich

GenElute™ Direct mRNA Miniprep Kits

sufficient for 10 purifications

Synonym(e):

GenElute™ Direct mRNA Miniprep Kit, GenElute™ mRNA Kit, Gen Elute

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About This Item

UNSPSC-Code:
41105501
NACRES:
NA.52

Verwendung

sufficient for 10 purifications

Qualitätsniveau

200

Methode(n)

RNA purification: suitable

Lagertemp.

15-25°C

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Allgemeine Beschreibung

The GenElute™ Direct mRNA Miniprep kit provides a convenient format to isolate polyadenylated mRNA directly from mammalian cells and tissues. The direct mRNA isolation procedure is based on that of Badley. Up to 107 mammalian cells or 40 mg tissue are lysed and homogenized, either with the filtration columns provided or with
a mechanical homogenizer. RNase is eliminated during a 10 minute proteinase K digestion. Sodium chloride is added, and polyadenylated RNA is captured on oligo(dT) polystyrene beads during a 10 minute incubation. For further enrichment, RNA may be released from the beads into fresh lysis solution and recaptured with the original beads. After 3 washes in a spin column, purified mRNA is eluted in 100 μl of 10 mM Tris-HCl, pH 7.4.

Anwendung

The GenElute Direct Kit mRNA kit provides convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues.

Leistungsmerkmale und Vorteile

  • mRNA captured on oligo(dT) polystyrene beads in 10 minutes, with no mixing or rocking (Fig. 1)
  • Poly (A)+ mRNA isolated from total RNA in 40 minutes (Fig. 2) or 60 minutes directly from cells and tissues (Fig. 3)
  • Oligo(dT) polystyrene beads require fewer wash steps

Sonstige Hinweise

Use for isolating mRNA directly from mammalian cells or tissues.
For additional information, please see www.sigma-aldrich.com/mrna.

Rechtliche Hinweise

GenElute is a trademark of Sigma-Aldrich Co. LLC

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • Elution solution 1.5 mL
  • Filtration columns with tubes 10 ea
  • Lysis solution 20 mL
  • 5 M NaCl 1.5 mL
  • Oligo(dT)-polystyrene beads .3 mL
  • Proteinase K 5 mg
  • 40% Glycerol solution .6 mL
  • Spin columns with tubes 10 ea
  • Collection tube 10 ea
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Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Brahmchetna Singh et al.
Molecular cancer, 6, 82-82 (2007-12-26)
Retinoids are potent growth inhibitory and differentiating agents in a variety of cancer cell types. We have shown that retinoids induce growth arrest in all pancreatic cancer cell lines studied, regardless of their p53 and differentiation status. However, the mechanism

Protokolle

Reverse Transcription Using ReadyScript cDNA Synthesis (RDRT)

Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.

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