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Merck

D8899

Sigma-Aldrich

Desoxyribonukleinsäure, einsträngig aus Kalbsthymus

lyophilized powder

Synonym(e):

DNA einzelsträngig

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About This Item

CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52

Qualität

for molecular biology

Qualitätsniveau

Form

lyophilized powder

Mol-Gew.

~50 kb

Zusammensetzung

ssDNA, ≥65%

Lagertemp.

−20°C

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Allgemeine Beschreibung

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Anwendung

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Leistungsmerkmale und Vorteile

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Menge

One mg DNA is approx. 25 A260 units.

Angaben zur Herstellung

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Rekonstituierung

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

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Lagerklassenschlüssel

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WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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J A Hansen et al.
Journal of animal science, 75(7), 1810-1821 (1997-07-01)
We evaluated the effects of recombinant porcine somatotropin (pST) and the beta-adrenergic agonist salbutamol on plasam endocrine and metabolite profiles and muscle chemistry in three genotypes of growing barrows (n = 96, 139 d old). Treatments were in a 2
H Kamei
Cell biology international, 20(11), 769-775 (1996-11-01)
The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were
José Luis García-Giménez et al.
Journal of inorganic biochemistry, 121, 167-178 (2013-02-07)
Ternary copper(II) complexes [Cu(NST)2(phen)] (1) and [Cu(NST)2(NH3)2]·H2O (2) [HNST=N-(4,5-dimethylthiazol-2-yl)naphthalene-1-sulfonamide] were prepared and characterized by physico-chemical techniques. Both 1 and 2 were structurally characterized by X-ray crystallography. The crystal structures show the presence of a distorted square planar CuN4 geometry in
DNA-cellulose chromatography.
Alberts, B., and Herrick, G.
Methods in Enzymology, 21, 198-217 (1971)
Inessa Halets et al.
International journal of pharmaceutics, 454(1), 1-3 (2013-07-09)
In most articles, cytotoxicity of cationic polyamidoamine (PAMAM) dendrimers toward red blood cells has been exclusively explained by their surface charge. We have focused on dendrimer hydrophobicity as a second possible factor that determines this cytotoxicity. Using PAMAM-NH2 dendrimers from

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