D8899

Desoxyribonukleinsäure, einsträngig aus Kalbsthymus

lyophilized powder

• Synonym(e): DNA einzelsträngig
• CAS-Nummer: 91080-16-9
• EG-Nummer: 293-507-3
• MDL-Nummer: MFCD00163436
• UNSPSC-Code: 41106310
• eCl@ss: 32160414
• NACRES: NA.52

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 200
• Form: lyophilized powder
• Mol-Gew.: ~50 kb
• Zusammensetzung: ssDNA, ≥65%
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Anwendung

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Leistungsmerkmale und Vorteile

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Menge

One mg DNA is approx. 25 A₂₆₀ units.

Angaben zur Herstellung

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Rekonstituierung

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves