D2916

Lambda DNA Mixed Digest

for DNA electrophoresis

• UNSPSC-Code: 12352200
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 100; 200
• Form: buffered aqueous solution
• Verwendung: 100 uses (0.1 mg size); 20 uses (20 μg size)
• Eignung: suitable for electrophoresis (DNA)
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Anwendung

Lambda DNA Mixed Digest has been used for DNA electrophoresis.

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Komponenten

Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Angaben zur Herstellung

Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.

Sonstige Hinweise

For optimal resolution, the recommended agarose gel concentration is 0.4%.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable