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D2321

Sigma-Aldrich

DAF-FM DA solution

5 mM in DMSO, ≥97% (HPLC)

Synonym(e):

4-Amino-5-methylamino- 2′,7′-difluorofluorescein diacetate, Diaminofluorescein-FM diacetate

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About This Item

Empirische Formel (Hill-System):
C25H18F2N2O7
CAS-Nummer:
254109-22-3
Molekulargewicht:
496.42
UNSPSC-Code:
12352200
NACRES:
NA.77

Qualitätsniveau

100

Assay

≥97% (HPLC)

Form

DMSO solution

Lagerbedingungen

desiccated
protect from light

Farbe

clear colorless

Versandbedingung

wet ice

Lagertemp.

−20°C

InChI

1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChIKey

BEVHTVRRVVEMEF-UHFFFAOYSA-N

Biochem./physiol. Wirkung

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

Leistungsmerkmale und Vorteile

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Sonstige Hinweise

Light sensitive

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Aurora Parlati et al.
Frontiers in plant science, 8, 1299-1299 (2017-08-10)
The PII protein in plants has been associated to many different tissue specialized roles concerning the Nitrogen assimilation pathways. We report here the further characterization of
Diana Passaro et al.
Cancer cell, 32(3), 324-341 (2017-09-06)
The biological and clinical behaviors of hematological malignancies can be influenced by the active crosstalk with an altered bone marrow (BM) microenvironment. In the present study, we provide a detailed picture of the BM vasculature in acute myeloid leukemia using
Gen Li et al.
Frontiers in plant science, 11, 1215-1215 (2020-09-10)
Anthocyanin-derived fleshy fruit pigmentation has become an excellent system for studying the regulatory network underlying fruit ripening and quality. The transcriptional control of anthocyanin biosynthesis by MYB-bHLH-WDR complexes has been well established, but the intermediate signals through which the environmental
Caihua Wang et al.
Stem cell research & therapy, 10(1), 175-175 (2019-06-15)
Cancer stem cells (CSCs) play a critical role in tumor development and progression and are involved in cancer metastasis. The role of reactive oxygen species (ROS) in CSCs and cancer metastasis remains controversial. The aim of the present study was
Diego Piacentini et al.
Environment international, 132, 105094-105094 (2019-09-05)
Over the last years, various acellular assays have been used for the evaluation of the oxidative potential (OP) of particular matter (PM) to predict PM capacity to generate reactive oxygen (ROS) and nitrogen (RNS) species in biological systems. However, relationships

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