COR-L23/R23-

96042337, human unknown/unspecified, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: COR-L23/R23-, 96042337
• Biologische Quelle: human unknown/unspecified
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian lung large cell carcinoma,drug-resistant revertant

Beschreibung der Zelllinie

The revertant lung cancer cell line COR-L23/R23- was generated by growing the doxorubicin-selected, resistant variant COR-L23/R (Sigma Catalogue number. 96042339) without drug exposure for 24-28 weeks. The cell line, also known as COR-L23/Rev, overexpresses multidrug resistance -associated protein (MRP), but to a lesser extent than the parent line. It was shown that the cell line has the ability to recover quickly, similar levels of MRP expression and resistance as COR-L23/R after a transient exposure to the MDR-drugs doxorubicin and vincristine.

Anwendung

Studies on lung cancer, drug-resistance analysis

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,11
D16S539: 11,13
D5S818: 10,12
D7S820: 9
THO1: 9
TPOX: 8,11
vWA: 19

Nährmedium

RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Sonstige Hinweise

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