OV56

96020759, human ovary, Epithelial

• Synonym(e): OV-56 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: OV56, 96020759
• Biologische Quelle: human ovary
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: VEGF
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human ovarian serous carcinoma (ascites) stage IV, poorly differentiated

Beschreibung der Zelllinie

The OV56 cell line was established from ascitic fluid derived from a 46-year-old female patient diagnosed with ovarian cancer. The tumour was characterised as stage IV, poorly differentiated. Immunocytochemical staining of the cells was reported to show strong staining for cytokeratin (CK), weak staining for carcinoembryonic antigen (CEA) and strong staining for human milk fat globulin (HMFG-2). Production of vascular endothelial growth factor (VEGF) has been detected by ELISA. The patient had received sequential treatment with cisplatin and cyclophosphamide, cisplatin and etoposide and subsequently tamoxifen.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 10,13
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 9,11
vWA: 15,19

Nährmedium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) + 0.5 ug/ml hydrocortisone + 10ug/ml insulin

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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