M15

95102517, mouse kidney, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: M15, 95102517
• Biologische Quelle: mouse kidney
• Wachstumsmodus: Adherent
• Karyotyp: Hyperdiploid
• Morphologie: Epithelial
• Produkte: WT1 (expressed gene)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse mesonephric epithelium, polyoma virus large T transformed

Beschreibung der Zelllinie

The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).

Anwendung

Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. NB: Cell growth is impaired at lower densities.

Sonstige Hinweise

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