DMS 454

95062832, human lung, Polygonal

• Synonym(e): DMS454 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: DMS 454, 95062832
• Biologische Quelle: human lung
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Polygonal
• Produkte: Calcitonin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian lung, small cell carcinoma

Beschreibung der Zelllinie

The small cell carcinoma DMS 454 was isolated in 1981 from lung tissue of a male patient at time of autopsy. The cells contain dense-core vesicles and secrete calcitonin.

Anwendung

Human lung tumour model, hormone secretion

Nährmedium

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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