MES-SA

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95051030, human uterus, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human uterus
• Verpackung: tube of 5 μg 95051030-DNA-5UG; pkg of vial of cells 95051030-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 45, monosomic forms of chromosomes 5, 6 and 7
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human uterus sarcoma

Beschreibung der Zelllinie

A human uterine sarcoma derived in 1980 from a tumour obtained at the time of a hysterectomy from a 56 year old Caucasian female. The cells form tumours in athymic nude mice, readily form colonies in soft agar and have a reported doubling time of 22-24 hours. The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin. They are resistant to vinblastine dacarbazine, cisplatin, melphalan, vincristine methotrexate and etoposide. The multi drug-resistant cell line MES-SA/Dx5 was established from MES-SA cells grown in the presence of doxorubicin.

Anwendung

Tumourigenicity and chemotherapeutic agent studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
TPOX: 8,11
vWA: 18

Nährmedium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm² using 0.03% EDTA; 5% CO₂; 37°C. Do not use trypsin.

Sonstige Hinweise

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