DBTRG.05MG

93061119, human brain, Glial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: DBTRG.05MG, 93061119
• Biologische Quelle: human brain
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 87-91, hypotetraploid
• Morphologie: Glial
• Produkte: Not specified
• Rezeptoren: Epidermal growth factor (EGF) R
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human glioblastoma

Beschreibung der Zelllinie

Derived from an adult female with glioblastoma multiforme treated with local brain irradiation and multi drug chemotherapy. By flow cytometry these cells were almost 100% positive for MHC Class I.

Anwendung

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 12
D5S818: 12,13
D7S820: 11
THO1: 8
TPOX: 8
vWA: 15,16

Nährmedium

RPMI 1640 + 2mM Glutamine + 1% HT + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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