ND-C

92090913, mouse nerve, Neuronal

• UNSPSC-Code: 41106514

Eigenschaften

• product name: ND-C, 92090913
• Biologische Quelle: mouse nerve
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Neuronal
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse neuroblastoma x Rat neurone hybrid

Beschreibung der Zelllinie

ND-C hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-gauglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300) derived azaguanine resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express rat Thy 1.1 marker and the sensory neuropeptide substance P. The cell line has been mycoplasma eradicated at ECACC.

Anwendung

Study of neuronal differentiation, cell adhesion and expression of neuronal proteins.

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3 x 10,000 cells/cm²; aspirate or gently tap cells loose, use of trypsin not necessary; 5% CO₂; 37°C.

Sonstige Hinweise

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