ND8/34 Cell Line from mouse

92090904, Mouse x Rat Hybridoma nerve, Neuronal

• UNSPSC-Code: 41106514

Eigenschaften

• product name: ND8/34 Cell Line from mouse, 92090904
• Biologische Quelle: Mouse x Rat Hybridoma nerve
• Beschreibung: Mouse neuroblastoma x Rat neurone hybrid
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Neuronal
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse neuroblastoma x Rat neurone hybrid

Beschreibung der Zelllinie

The hybrid ND8/34 is a subclone of ND8 which was derived after fusion of primary neonatal rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. ND8/34 are electrically excitable to the chemical activator of nociceptive sensory neurons, bradykinin.

Anwendung

Study of neuronal differentiation.

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.