CFPAC-1

91112501, human pancreas (liver metastasis), Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: CFPAC-1, 91112501
• Biologische Quelle: human pancreas (liver metastasis)
• Wachstumsmodus: Adherent
• Karyotyp: Hyperdiploid, (65-75 chromosomes)
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Cystic fibrosis trans-membrane regulator (CFTR)
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): metastasis
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian pancreatic adenocarcinoma

Beschreibung der Zelllinie

CFPAC-1 is a ductal pancreatic adenocarcinoma derived by differential trypsinisation of explant cultures from a metastatic lesion in the liver of a 26 year old caucasian male with cystic fibrosis (CF). The cell line expresses cystic fibrosis trans-membrane regulator (CFTR) and ion transport properties consistent with the CF defect. CFPAC-1 cells show an epithelial morphology and polarisation with apical microvilli. Expression of cytokeratin and oncofoetal antigens characteristic of pancreatic duct cells have been described. The cells express the CF gene and show the most common CF mutation, deletion of three nucleotides result in a phenylalanine-508 deletion. CFPAC-1 cells are also tumorigenic in nude mice.

Anwendung

Cystic fibrosis studies, genetic analysis

Nährmedium

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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