MLg

90112707, mouse lung, Fibroblast-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: MLg, 90112707
• Biologische Quelle: mouse lung
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 64; hypodiploid
• Morphologie: Fibroblast-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse ddY lung

Beschreibung der Zelllinie

Established from normal lung tissue of a new-born, ddY strain mouse. Both induced and spontaneous release of C-type particles have been reported.

Anwendung

Virus studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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