TT

92050721, human thyroid, Not specified

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: TT, 92050721
• Biologische Quelle: human thyroid
• Wachstumsmodus: Adherent
• Karyotyp: Aneuploid karyotype with marker chromosomes
• Morphologie: Not specified
• Produkte: Calcitonin, Carcinoembryonic antigen (CEA)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian thyroid medullary carcinoma

Beschreibung der Zelllinie

Established from a thyroid medullary carcinoma of a 77 year old Caucasian female. Tumourigenic in nude mice.

Anwendung

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 16,18

Nährmedium

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Population doubling is very slow, cells can take up to 5 days to reach 50% confluency; media change 2-3 times a week is recommen

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